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The Coler Lab is a dynamic team of translational researchers focused on understanding the complex pathogen-host interactions that control the outcome of infection and immunity. The laboratory is dedicated to characterizing vaccines, phages, and host-directed therapies against a variety of epidemic and pandemic diseases, including tuberculosis (TB) and nontuberculous mycobacteria (NTM). The laboratory is a member of the Center for Global Infectious Disease Research at the Seattle Children’s Research Institute (SCRI), is a lead component of the Immune Mechanisms of Protection Against Mycobacterium Tuberculosis (IMPAc-TB) consortium, is an endpoint lab for the Infectious Disease Clinical Research Consortium (IDCRC), and is one of 9 Vaccine Treatment and Evaluation Units (VTEUs) within the IDCRC consortium that designs and conducts clinical trials to evaluate promising vaccines, treatments, and devices for infectious disease, each funded by the National Institutes of Health (NIH). The Coler Lab ( https://www.seattlechildrens.org/research/centers-programs/global-infectious-disease-research/research-areas-and-labs/coler-lab/ ) is recruiting a Research Associate to contribute to one or more projects that involve in vivo infectious disease models, processing of human blood and tissue samples, and performing an array of microbiological and immunological assays. The successful candidate will be committed to SCRI’s mission of turning discoveries into lifesaving therapies, be a strong team player capable of working with moderate supervision and committed to learning new skills. A strong background in immunology and flow cytometry are preferred.

Responsibilities:

• Animal work including injections via multiple routes, blood collection, dissections, tissue collection, monitoring temperatures, weights in mice under BSL2/BSL3 conditions

• Perform specimen processing, and shipping for domestic and international sites

• Contributes to maintenance of the sample repository including sample tracking procedures

• Perform intracellular cytokine staining/multi-color flow cytometry, antibody neutralization, ELISPOTS, ELISA and other immunological assays

• Infecting mice under BSL2/3 conditions

• Enumeration of nontuberculous mycobacteria and/or Mycobacterium tuberculosis from experimental animals by culture under BSL3 conditions

• Perform plaque assays to enumerate mycobacteriophages

• Prepare blood and tissues for ex vivo assays under BSL2/BSL3 conditions

• Monitors laboratory supply inventories and works with purchasing to ensure available stocks

• Communicates with research scientists and management on a regular basis

• Utilize mathematical and scientific techniques to compile and analyze data

• Maintain the highest standards of laboratory practice

• Strong organizational and mathematical skills (e.g. dilutions, conversions, overage calculations)

• Close attention to detail including recording observations and findings in audited lab notebooks following established guidelines

• Excellent oral and written communication skills

• Proficiency in MS Office and scientific data/graphics/statistical programs

• Proficient in trouble-shooting experiments and maintenance of equipment

• Ability to multi-task and perform multiple scientific protocols and procedures under direction

• Ability to maintain confidentiality of sensitive information

• Takes initiative to schedule work and has excellent time management skills

• Possess a work ethic that includes neatness, punctuality, and accuracy

• Must be self-motivated, organized and be able to work in a team environment

• Enjoys learning new concepts or technical skills

• Does not require travel outside of Seattle

• Requires infrequent evening or weekend work

Required Education and Experience

Bachelor’s Degree in Microbiology, Biochemistry, Immunology, or related field. At least five (5) years laboratory experience.

Required Credentials

N/A.

Preferred

Master’s Degree in Microbiology, Biochemistry, Immunology, or related field. Previous research experience in biochemistry, molecular biology, and tissue culture techniques.

Compensation Range

$28.44 - $42.66 per hour

Salary Information

This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.

Disclaimer for Out of State Applicants

This compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors .

Benefits Information

Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website www.seattlechildrens.org/careers/benefits .

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

Our founding promise to the community is as valid today as it was over a century ago: we will care for all children in our region, regardless of the families’ ability to pay. Together, we deliver superior patient care, advance new discoveries and treatments through pediatric research, and serve as the pediatric and adolescent, academic medical center for Washington, Alaska, Montana and Idaho – the largest region of any children’s hospital in the country.

In 2022, U.S. News & World Report once again ranked Seattle Children’s among the nation’s best children’s hospitals – for the 30th year in a row. For more than a decade, Seattle Children’s has been nationally ranked in all 10 specialty areas evaluated by U.S. News & World Report. We are honored to be the top-ranked pediatric hospital in Washington and the Pacific Northwest.

As a Magnet designated institution, and classified among America’s best large employers by Forbes, we recognize the importance of hiring and developing great talent to provide best-in-class care to the patients and families we serve. Our organizational DNA takes form in our core values: Compassion, Excellence, Integrity, Collaboration, Equity and Innovation. Whether it’s delivering frontline care to our patients in a kind and caring manner, practicing the highest standards of quality and safety, or being relentlessly curious as we work towards eradicating childhood diseases, these values are the fabric of our culture and community. The future starts here.

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.

Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.