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Reference #: 24001387 Description Grade: T24 The link below will give you information about the University's "T" salary structure. Learn more about the "T" salary structure. A variety of important factors are reviewed by HR when considering salary, including job duties, the applicant's education and experience, all relevant internal equity considerations, department budget, and funding source. The Assistant Scientist under the close supervision of the PI in the Lewis Katz School of Medicine's Shriners Pediatric Research Center, will conduct research activities involving mouse genetics, recombinant DNA techniques, and histological and immunohistochemical analyses according to the lab research protocols. This individual will be actively involved in ongoing research in the lab with the close supervision of the PI. Responsibilities include: Primarily responsible for mouse handling, colony management and maintenance, PCR-based genotyping, tissue dissection, histological, immunohistochemical and ultrastructural assays and microscopic imaging using bright-field, fluorescent and confocal microscopes. Independently perform mouse survival surgery, primary neuronal cultures, cell transfection and research data collection/analysis; Molecular biological techniques, such as PCR, qPCR, protein biochemistry (such as Western blotting), flow cytometry - cell preparation, fluorescent labeling and data acquisition/analysis; Making commonly used reagents, buffers, plates, etc. Maintain laboratory notebook with precise experimental records. Perform other duties as assigned by the PI. Required Education and Experience: Bachelor's degree in a related area of study and at least 2 years related research experience. An equivalent combination of education and experience may be considered. Required Skills and Abilities: *Demonstrated ability to independently perform Western Blot analysis, mouse genotyping; *Demonstrated proficiency in recombinant DNA technology including DNA preparation, polymerase chain reaction (PCR), ligation/transformation and subcloning. *Demonstrated proficiency in immunohistochemistry, confocal microscopy, electron microscopy and imaging program such as Imaris. *Demonstrated ability to operate all standard laboratory and office equipment including MS Office and Adobe photoshop. *Demonstrated proficiency in working with excel spreadsheets for database. *Demonstrated ability to work independently. *Ability to perform multiple tasks simultaneously. *Ability to quickly learn new techniques. *Excellent technical and analytical skills. *Ability to work in a team environment. *Strong written and oral communication skills. This is a grant funded position Temple University is committed to a policy of equal opportunity for all in every aspect of its operations, including employment, service, and educational programs. The University has pledged not to discriminate on the basis of age, color, disability, marital status, national origin or ethnic origin, race, religion, sex (including pregnancy), sexual orientation, gender identity, genetic information or veteran status. Compliance Statement: In the performance of their functions as detailed in the position description employees have an obligation to avoid ethical, legal, financial and other conflicts of interest to ensure that their actions and outside activities do not conflict with their primary employment responsibilities at the institution. Employees are also expected to understand and be in compliance with applicable laws, University and employment policies and regulations, including NCAA regulations for areas and departments which their essential functions cause them to interact. To obtain additional information about Temple University please visit our website at www.temple.edu. Temple University's Annual Security and Fire Safety Report contains statistics, policies, and procedures related to campus safety. Click here. You may request a copy of the repor by calling Temple University's Campus Safety Services at 215-204-7900.

Primary Location:Pennsylvania-Philadelphia-Health Sciences Campus (HSC)-Medical Education and Research Building

Job:Staff Schedule:Full-time Shift:Day Job Employee Status:Regular

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