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The Modell lab at Johns Hopkins School of Medicine studies CRISPR-Cas9 biology and bacterial mechanisms of phage defense more broadly. Specifically, we explore how CRISPR-Cas9 systems function in nature to provide their bacterial hosts with adaptive immunity against phages and plasmids. More recently, we have begun identifying and characterizing new phage defense systems, and we believe this knowledge will be crucial for the development of phage therapies for antibiotic-resistant bacterial infections. We ask fundamental biological questions with an array of approaches including genetics, genomics, molecular biology, biochemistry, bioinformatics and live-cell microscopy. Our studies provide insights into (i) bacterial decision-making and stress responses, (ii) the emerging field of phage therapy for antibiotic resistant bacterial infections and (iii) the development of new and improved Cas9 tools.

We are seeking a Research Technologist to carry out research projects (85% of time) under supervision of the PI or a senior member of the lab and to provide general support to the laboratory (15% of time), including managing equipment and supplies. The lab is located in Baltimore, MD at Johns Hopkins University School of Medicine in the department of Molecular Biology and Genetics. Please visit our website for additional information: http://www.modelllab.com/.

Specific Duties and Responsibilities:

• Follows established protocols and uses aseptic technique to perform laboratory tests and experiments which may include: propagating and manipulating bacterial cell cultures and bacteriophages, cloning and sequencing plasmid constructs, performing molecular biology techniques such as PCR, DNA gel electrophoresis and nucleic acids purification, preparation of DNA libraries for deep sequencing, Western and Northern blots, and/or human cell culture and Cas9 editing experiments.

• Keeps a detailed electronic laboratory notebook.

• Participates in journal clubs and develops skills in written and oral communication.

• Manages and organizes laboratory equipment, protocols, databases and inventory.

• Prepares specialized buffers and reagents to support all lab members.

• Complies with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.

• Actively seeks to increase skills and expertise.

• Performs miscellaneous related duties as assigned. For an example of our work, please see our recent paper (https://pubmed.ncbi.nlm.nih.gov/33421369/) or check out our website (http://www.modelllab.com) .

Minimum Qualifications

• Bachelor's Degree in Biology, Chemistry or related field.

Preferred Qualifications

• One (1) year of laboratory experience.

• Independent research project not related to normal course requirements preferred.

Classified Title: Research Technologist

Role/Level/Range: ACRO40/E/02/CC

Starting Salary Range: Min $15.5 - Max $26.00 HRLY ($40,000 targeted; Commensurate with experience)

Employee group: Full Time

Schedule: Monday-Friday 8:30am-5:00pm

Exempt Status: Non-Exempt

Location: School of Medicine Campus

Department name: SOM MBG Molecular Biology and Genetics

Personnel area: School of Medicine

Equal Opportunity Employer:

Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.